Using light scattering to evaluate the separation of polydisperse nanoparticles.

ABSTRACT: The analysis of natural and otherwise complex samples is challenging and yields uncertainty about the accuracy and precision of measurements. Here we present a practical tool to assess relative accuracy among separation protocols for techniques using light scattering detection. Due to the highly non-linear relationship between particle size and the intensity of scattered light, a few large particles may obfuscate greater numbers of small particles. Therefore, insufficiently separated mixtures may result in an overestimate of the average measured particle size. Complete separation of complex samples is needed to mitigate this challenge. A separation protocol can be considered improved if the average measured size is smaller than a previous separation protocol. Further, the protocol resultin

SUBMITTER: Galyean AA 

PROVIDER: S-EPMC4576494 | biostudies-literature | 2015 Jul

REPOSITORIES: biostudies-literature