Identification of Endoglin as an epigenetically regulated tumour-suppressor gene in lung cancer.

ABSTRACT:

Background

The transforming growth factor-beta (TGF- β) pathway has been implicated in proliferation, migration and invasion of various cancers. Endoglin is a TGF-β accessory receptor that modulates signalling. We identified Endoglin as an epigenetically silenced tumour-suppressor gene in lung cancer by means of a genome-wide screening approach, then sought to characterise its effect on lung cancer progression.

Methods

Methylation microarray and RNA sequencing were carried out on lung cancer cell lines. Epigenetic silencing of Endoglin was confirmed by methylation and expression analyses. An expression vector and a 20-gene expression panel were used to evaluate Endoglin function. Pyrosequencing was carried out on two independent cohorts comprising 112 and 202 NSCLC cases, re

SUBMITTER: O'Leary K 

PROVIDER: S-EPMC4578092 | biostudies-literature | 2015 Sep

REPOSITORIES: biostudies-literature