Longitudinal study of the early-life fecal and nasal microbiotas of the domestic pig.

ABSTRACT:

Background

The mammalian microbiota plays a key role in host health and disease susceptibility. However, knowledge of the early-age microbiota of pigs is lacking. The purpose of this study was to use high-throughput next-generation sequencing to characterize the fecal and nasal microbiotas of pigs during early life.

Results

Ten commercially-raised pigs were randomly enrolled at birth and sampled throughout the first 7 weeks of life. DNA was extracted from fecal and nasal samples and the hypervariable region V4 of the 16S rRNA gene was amplified. The product was sequenced using the Illumina MiSeq platform and 2 × 250 chemistry. Sequencing data was processed and analyzed with the mothur algorithms using an operational taxonomic unit approach. In total, 4.7 million and 5.4 mill

SUBMITTER: Slifierz MJ 

PROVIDER: S-EPMC4578254 | biostudies-literature | 2015 Sep

REPOSITORIES: biostudies-literature