Bioinformatic identification and expression analysis of Nelumbo nucifera microRNA and their targets.

ABSTRACT:

Premise of the study

Sacred lotus (Nelumbo nucifera) is a perennial aquatic herbaceous plant of ecological, ornamental, and economic importance. MicroRNAs (miRNAs) play an important role in plant development. However, reports of miRNAs and their role in sacred lotus have been limited.

Methods

Using the homology search of known miRNAs with genome and transcriptome contig sequences, we employed a pipeline to identify miRNAs in N. nucifera. We also predicted the targets of these miRNAs.

Results

We found 106 conserved miRNAs in N. nucifera, and 456 of their miRNA targets were annotated. Quantitative real-time PCR (qRT-PCR) analysis revealed the different expression levels of the 10 selected conserved miRNAs in tissues of young leaves, stems, and flowers of N. nucifera. Ne

SUBMITTER: Pan L 

PROVIDER: S-EPMC4578376 | biostudies-literature | 2015 Sep

REPOSITORIES: biostudies-literature