Clinical implementation of integrated whole-genome copy number and mutation profiling for glioblastoma.

ABSTRACT:

Background

Multidimensional genotyping of formalin-fixed paraffin-embedded (FFPE) samples has the potential to improve diagnostics and clinical trials for brain tumors, but prospective use in the clinical setting is not yet routine. We report our experience with implementing a multiplexed copy number and mutation-testing program in a diagnostic laboratory certified by the Clinical Laboratory Improvement Amendments.

Methods

We collected and analyzed clinical testing results from whole-genome array comparative genomic hybridization (OncoCopy) of 420 brain tumors, including 148 glioblastomas. Mass spectrometry-based mutation genotyping (OncoMap, 471 mutations) was performed on 86 glioblastomas.

Results

OncoCopy was successful in 99% of samples for which sufficient DNA wa

SUBMITTER: Ramkissoon SH 

PROVIDER: S-EPMC4578577 | biostudies-literature | 2015 Oct

REPOSITORIES: biostudies-literature