Depletion of yeast PDK1 orthologs triggers a stress-like transcriptional response.

ABSTRACT:

Background

Pkh proteins are the PDK1 orthologs in S. cerevisiae. They have redundant and essential activity and are responsible for the phosphorylation of several members of the AGC family of protein kinases. Pkh proteins have been involved in several cellular functions, including cell wall integrity and endocytosis. However the global expression changes caused by their depletion are still unknown.

Results

A doxycycline-repressible tetO7 promoter driving the expression of PKH2 in cells carrying deletions of the PKH1 and PKH3 genes allowed us to progressively deplete cells from Pkh proteins when treated with doxycycline. Global gene expression analysis indicate that depletion of Pkh results in the up-regulation of genes involved in the accumulation of glycogen and also of tho

SUBMITTER: Pastor-Flores D 

PROVIDER: S-EPMC4578605 | biostudies-literature | 2015 Sep

REPOSITORIES: biostudies-literature