Relationship between serum response factor and androgen receptor in prostate cancer.

ABSTRACT:

Background

Serum response factor (SRF) is an important transcription factor in castrate-resistant prostate cancer (CRPC). Since CRPC is associated with androgen receptor (AR) hypersensitivity, we investigated the relationship between SRF and AR.

Materials and methods

Transcriptional activity was assessed by luciferase assay. Cell proliferation was measured by MTT and flow cytometry. Protein expression in patients was assessed by immunohistochemistry.

Results

To investigate AR involvement in SRF response to androgen, AR expression was down-regulated using siRNA. This resulted in the abrogation of SRF induction post-DHT. Moreover, DHT stimulation failed to induce SRF transcriptional activity in AR-negative PC346 DCC cells, which was only restored following AR over-expre

SUBMITTER: Prencipe M 

PROVIDER: S-EPMC4579008 | biostudies-literature | 2015 Nov

REPOSITORIES: biostudies-literature