Identification of Host Cell Factors Associated with Astrovirus Replication in Caco-2 Cells.

ABSTRACT:

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Astroviruses are small, nonenveloped viruses with a single-stranded positive-sense RNA genome causing acute gastroenteritis in children and immunocompromised patients. Since positive-sense RNA viruses have frequently been found to replicate in association with membranous structures, in this work we characterized the replication of the human astrovirus serotype 8 strain Yuc8 in Caco-2 cells, using density gradient centrifugation and free-flow zonal electrophoresis (FFZE) to fractionate cellular membranes. Structural and nonstructural viral proteins, positive- and negative-sense viral RNA, and infectious virus particles were found to be associated with a distinct population of membranes separated by FFZE. The cellular proteins associated with this membrane population in in

SUBMITTER: Murillo A 

PROVIDER: S-EPMC4580174 | biostudies-literature | 2015 Oct

REPOSITORIES: biostudies-literature