De novo sequencing and assembly analysis of transcriptome in the Sodom apple (Calotropis gigantea).

ABSTRACT:

Background

The Sodom apple (Calotropis gigantea), a member of the Asclepiadaceae family, is a large evergreen shrub native to continental Asia and northern Africa. As an important medicinal shrub and a fiber resource plant, there is an urgent need for developing molecular markers to facilitate breeding and genetic improvement of varieties.

Results

In this study, using the Illumina high throughput sequencing technique we obtained about 45 million paired end sequencing reads, De novo assembled and generated a total of 133,634 transcripts with a mean of 1837.47 bp in length. Based on protein homology searches against available databases, a total of 21,851 unigenes were functionally annotated. In particular, many transcripts that encode for putative proteins involved in fiber an

SUBMITTER: Muriira NG 

PROVIDER: S-EPMC4580217 | biostudies-literature | 2015 Sep

REPOSITORIES: biostudies-literature