Gene networks and transcription factor motifs defining the differentiation of stem cells into hepatocyte-like cells.

ABSTRACT:

Background & aims

The differentiation of stem cells to hepatocyte-like cells (HLC) offers the perspective of unlimited supply of human hepatocytes. However, the degree of differentiation of HLC remains controversial. To obtain an unbiased characterization, we performed a transcriptomic study with HLC derived from human embryonic and induced stem cells (ESC, hiPSC) from three different laboratories.

Methods

Genome-wide gene expression profiles of ESC and HLC were compared to freshly isolated and up to 14days cultivated primary human hepatocytes. Gene networks representing successful and failed hepatocyte differentiation, and the transcription factors involved in their regulation were identified.

Results

Gene regulatory network analysis demonstrated that HLC represent a

SUBMITTER: Godoy P 

PROVIDER: S-EPMC4580233 | biostudies-literature | 2015 Oct

REPOSITORIES: biostudies-literature