Gene expression changes in the salivary glands of Anopheles coluzzii elicited by Plasmodium berghei infection.

ABSTRACT:

Background

Malaria is a devastating infectious disease caused by Plasmodium parasites transmitted through the bites of infected Anopheles mosquitoes. Salivary glands are the only mosquito tissue invaded by Plasmodium sporozoites, being a key stage for the effective parasite transmission, making the study of Anopheles sialome highly relevant.

Methods

RNA-sequencing was used to compare differential gene expression in salivary glands of uninfected and Plasmodium berghei-infected Anopheles coluzzii mosquitoes. RNA-seq results were validated by quantitative RT-PCR. The transmembrane glucose transporter gene AGAP007752 was selected for functional analysis by RNA interference. The effect of gene silencing on infection level was evaluated. The putative function and tertiary structur

SUBMITTER: Pinheiro-Silva R 

PROVIDER: S-EPMC4580310 | biostudies-literature | 2015 Sep

REPOSITORIES: biostudies-literature