Role in tumor growth of a glycogen debranching enzyme lost in glycogen storage disease.

ABSTRACT:

Background

Bladder cancer is the most common malignancy of the urinary system, yet our molecular understanding of this disease is incomplete, hampering therapeutic advances.

Methods

Here we used a genome-wide functional short-hairpin RNA (shRNA) screen to identify suppressors of in vivo bladder tumor xenograft growth (n = 50) using bladder cancer UMUC3 cells. Next-generation sequencing was used to identify the most frequently occurring shRNAs in tumors. Genes so identified were studied in 561 patients with bladder cancer for their association with stratification of clinical outcome by Kaplan-Meier analysis. The best prognostic marker was studied to determine its mechanism in tumor suppression using anchorage-dependent and -independent growth, xenograft (n = 20), and metabolo

SUBMITTER: Guin S 

PROVIDER: S-EPMC4580555 | biostudies-literature | 2014 Apr

REPOSITORIES: biostudies-literature