Spatial reorganization of telomeres in long-lived quiescent cells.

ABSTRACT:

Background

The spatiotemporal behavior of chromatin is an important control mechanism of genomic function. Studies in Saccharomyces cerevisiae have broadly contributed to demonstrate the functional importance of nuclear organization. Although in the wild yeast survival depends on their ability to withstand adverse conditions, most of these studies were conducted on cells undergoing exponential growth. In these conditions, as in most eukaryotic cells, silent chromatin that is mainly found at the 32 telomeres accumulates at the nuclear envelope, forming three to five foci.

Results

Here, combining live microscopy, DNA FISH and chromosome conformation capture (HiC) techniques, we report that chromosomes adopt distinct organizations according to the metabolic status of the cell.

SUBMITTER: Guidi M 

PROVIDER: S-EPMC4581094 | biostudies-literature | 2015 Sep

REPOSITORIES: biostudies-literature