Design of CID-cleavable protein cross-linkers: identical mass modifications for simpler sequence analysis.

ABSTRACT: The cross-linking Mass Spectrometry (XL-MS) technique has enormous potential for studying the interactions between proteins, and it can provide detailed structural information about the interaction interfaces in large protein complexes. Such information has been difficult to obtain by conventional structural methods. One of the primary impediments to the wider use of the XL-MS technique is the extreme challenge in sequencing cross-linked peptides because of their complex fragmentation patterns in MS. A recent innovation is the development of MS-cleavable cross-linkers, which allows direct sequencing of component peptides for facile identification. Sulfoxides are an intriguing class of thermally-cleavable compounds that have been shown to fragment selectively during low-energy collisional i

SUBMITTER: Kandur WV 

PROVIDER: S-EPMC4581996 | biostudies-literature | 2015 Oct

REPOSITORIES: biostudies-literature