Introduction of germline residues improves the stability of anti-HIV mAb 2G12-IgM.

ABSTRACT: Immunoglobulins M (IgMs) are gaining increasing attention as biopharmaceuticals since their multivalent mode of binding can give rise to high avidity. Furthermore, IgMs are potent activators of the complement system. However, they are frequently difficult to express recombinantly and can suffer from low conformational stability. Here, the broadly neutralizing anti-HIV-1 antibody 2G12 was class-switched to IgM and then further engineered by introduction of 17 germline residues. The impact of these changes on the structure and conformational stability of the antibody was then assessed using a range of biophysical techniques. We also investigated the effects of the class switch and germline substitutions on the ligand-binding properties of 2G12 and its capacity for HIV-1 neutralization. Our r

SUBMITTER: Chromikova V 

PROVIDER: S-EPMC4582045 | biostudies-literature | 2015 Oct

REPOSITORIES: biostudies-literature