Profiling post-translational modifications of histones in human monocyte-derived macrophages.

ABSTRACT:

Background

Histones and their post-translational modifications impact cellular function by acting as key regulators in the maintenance and remodeling of chromatin, thus affecting transcription regulation either positively (activation) or negatively (repression). In this study we describe a comprehensive, bottom-up proteomics approach to profiling post-translational modifications (acetylation, mono-, di- and tri-methylation, phosphorylation, biotinylation, ubiquitination, citrullination and ADP-ribosylation) in human macrophages, which are primary cells of the innate immune system. As our knowledge expands, it becomes more evident that macrophages are a heterogeneous population with potentially subtle differences in their responses to various stimuli driven by highly complex epigene

SUBMITTER: Olszowy P 

PROVIDER: S-EPMC4582717 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature