Keratin and S100 calcium-binding proteins are major constituents of the bovine teat canal lining.

ABSTRACT: The bovine teat canal provides the first-line of defence against pathogenic bacteria infecting the mammary gland, yet the protein composition and host-defence functionality of the teat canal lining (TCL) are not well characterised. In this study, TCL collected from six healthy lactating dairy cows was subjected to two-dimensional electrophoresis (2-DE) and mass spectrometry. The abundance and location of selected identified proteins were determined by western blotting and fluorescence immunohistochemistry. The variability of abundance among individual cows was also investigated. Two dominant clusters of proteins were detected in the TCL, comprising members of the keratin and S100 families of proteins. The S100 proteins were localised to the teat canal keratinocytes and were particularly pr

SUBMITTER: Smolenski GA 

PROVIDER: S-EPMC4582823 | biostudies-literature | 2015 Sep

REPOSITORIES: biostudies-literature