Rapid detection of Salmonella enterica serovar Typhimurium DT104 strains by the polymerase chain reaction.

ABSTRACT:

Background

Infection with Salmonella enterica is a major public health concern in developed countries, and multidrug-resistant strains have become increasingly prevalent. S. enterica serovar Typhimurium DT104 (DT104) strains are prevalent in livestock in Japan and include numerous strains of multidrug-resistant S. enterica. Epidemiological analysis of these strains is critical for both agriculture and public health; however, diagnostic tests for these strains have yielded inconsistent results.

Results

We developed a rapid, simple, and inexpensive polymerase chain reaction test to detect multi-drug resistant DT104 strains. We designed primers specific to the prophage ST104 sequence encoded by DT104 strains and assessed the specificity of these primers by assaying a panel of 5

SUBMITTER: Yukawa S 

PROVIDER: S-EPMC4583067 | biostudies-literature | 2015 Sep

REPOSITORIES: biostudies-literature