Development of real-time PCR assay for detection of porcine circovirus-like virus P1 in domestic pigs in China.

ABSTRACT:

Background

The porcine circovirus-like agent P1 is a newly discovered DNA virus with a single-stranded circular genome that is highly homologous to that of porcine circovirus type 2. P1 infection can cause symptoms resembling postweaning multisystemic wasting syndrome. This study aims to develop a rapid, sensitive and specific method to detect P1.

Results

A pair of primers was designed and used to amplify a 119 bp DNA fragment to generate a recombinant plasmid which was served as the standard. A SYBR I qPCR protocol was established using the P1 recombinant plasmid standard and the sensitivity, specificity and stability of this method was analyzed. The results demonstrate a strong correlation with P1 recombinant plasmid titers when virus DNA copy numbers fall in between 10(0)

SUBMITTER: He KW 

PROVIDER: S-EPMC4583164 | biostudies-literature | 2015 Sep

REPOSITORIES: biostudies-literature