Exploring the rice dispensable genome using a metagenome-like assembly strategy.

ABSTRACT:

Background

The dispensable genome of a species, consisting of the dispensable sequences present only in a subset of individuals, is believed to play important roles in phenotypic variation and genome evolution. However, construction of the dispensable genome is costly and labor-intensive at present, and so the influence of the dispensable genome in genetic and functional genomic studies has not been fully explored.

Results

We construct the dispensable genome of rice through a metagenome-like de novo assembly strategy based on low-coverage (1-3×) sequencing data of 1483 cultivated rice (Oryza sativa L.) accessions. Thousands of protein-coding genes are successfully assembled, including most of the known agronomically important genes absent from the Nipponbare rice reference g

SUBMITTER: Yao W 

PROVIDER: S-EPMC4583175 | biostudies-literature | 2015 Sep

REPOSITORIES: biostudies-literature