All-In-One: Advanced preparation of Human Parenchymal and Non-Parenchymal Liver Cells.

ABSTRACT:

Background & aims

Liver cells are key players in innate immunity. Thus, studying primary isolated liver cells is necessary for determining their role in liver physiology and pathophysiology. In particular, the quantity and quality of isolated cells are crucial to their function. Our aim was to isolate a large quantity of high-quality human parenchymal and non-parenchymal cells from a single liver specimen.

Methods

Hepatocytes, Kupffer cells, liver sinusoidal endothelial cells, and stellate cells were isolated from liver tissues by collagenase perfusion in combination with low-speed centrifugation, density gradient centrifugation, and magnetic-activated cell sorting. The purity and functionality of cultured cell populations were controlled by determining their morphology, dis

SUBMITTER: Werner M 

PROVIDER: S-EPMC4583235 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature