Transcriptome and H3K27 tri-methylation profiling of Ezh2-deficient lung epithelium.

ABSTRACT: The adaptation of the lungs to air breathing at birth requires the fine orchestration of different processes to control lung morphogenesis and progenitor cell differentiation. However, there is little understanding of the role that epigenetic modifiers play in the control of lung development. We found that the histone methyl transferase Ezh2 plays a critical role in lung lineage specification and survival at birth. We performed a genome-wide transcriptome study combined with a genome-wide analysis of the distribution of H3K27 tri-methylation marks to interrogate the role of Ezh2 in lung epithelial cells. Lung cells isolated from Ezh2-deficient and control mice at embryonic day E16.5 were sorted into epithelial and mesenchymal populations based on EpCAM expression. This enabled us to dissec

SUBMITTER: Holik AZ 

PROVIDER: S-EPMC4583701 | biostudies-literature | 2015 Sep

REPOSITORIES: biostudies-literature