Determination of the Genetic Diversity of Different Bioluminescent Bacteria by Pulsed-Field Gel Electrophoresis (PFGE).

ABSTRACT:

Background

There are 4 different genera (i.e. Vibrio, Aliivibrio, Photobacterium, and Shewanella) in the new classification of bioluminescent bacteria. The mechanism of bioluminescence has yet to be fully elucidated. Therefore, the determination of physiological and genetic characteristics of bioluminescent bacteria isolated from different sources is very important. Pulsed-Field Gel Electrophoresis (PFGE) has the highest discriminatory power among the different molecular typing methods for the investigation of the clonal relationships between bacteria. For the PFGE analysis of bioluminescent bacteria, the NotI-HF™ is the method of choice among the restriction enzymes.

Objectives

The present study aimed to determine genetic relatedness via PFGE in 41 bioluminescent bacteria (

SUBMITTER: Ersoy Omeroglu E 

PROVIDER: S-EPMC4584076 | biostudies-literature | 2015 Jul

REPOSITORIES: biostudies-literature