Recombinant protein production facility for fungal biomass-degrading enzymes using the yeast Pichia pastoris.

ABSTRACT: Filamentous fungi are the predominant source of lignocellulolytic enzymes used in industry for the transformation of plant biomass into high-value molecules and biofuels. The rapidity with which new fungal genomic and post-genomic data are being produced is vastly outpacing functional studies. This underscores the critical need for developing platforms dedicated to the recombinant expression of enzymes lacking confident functional annotation, a prerequisite to their functional and structural study. In the last decade, the yeast Pichia pastoris has become increasingly popular as a host for the production of fungal biomass-degrading enzymes, and particularly carbohydrate-active enzymes (CAZymes). This study aimed at setting-up a platform to easily and quickly screen the extracellular express

SUBMITTER: Haon M 

PROVIDER: S-EPMC4585289 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature