New Details of the Human Corneal Limbus Revealed With Second Harmonic Generation Imaging.

ABSTRACT:

Purpose

To report novel findings of the human corneal limbus by using second harmonic generation (SHG) imaging.

Methods

Corneal limbus was imaged by using an inverted two-photon excitation fluorescence microscope. Laser (Ti:Sapphire) was tuned at 850 nm for two-photon excitation. Backscatter signals of SHG and autofluorescence (AF) were collected through a 425/30-nm emission filter and a 525/45-emission filter, respectively. Multiple, consecutive, and overlapping image stacks (z-stack) were acquired for the corneal limbal area.

Results

Two novel collagen structures were revealed by SHG imaging at the limbus: an anterior limbal cribriform layer and presumed anchoring fibers. Anterior limbal cribriform layer is an intertwined reticular collagen architecture just beneath

SUBMITTER: Park CY 

PROVIDER: S-EPMC4585533 | biostudies-literature | 2015 Sep

REPOSITORIES: biostudies-literature