Highly efficient CRISPR/Cas9-mediated transgene knockin at the H11 locus in pigs.

ABSTRACT: Transgenic pigs play an important role in producing higher quality food in agriculture and improving human health when used as animal models for various human diseases in biomedicine. Production of transgenic pigs, however, is a lengthy and inefficient process that hinders research using pig models. Recent applications of the CRISPR/Cas9 system for generating site-specific gene knockout/knockin models, including a knockout pig model, have significantly accelerated the animal model field. However, a knockin pig model containing a site-specific transgene insertion that can be passed on to its offspring remains lacking. Here, we describe for the first time the generation of a site-specific knockin pig model using a combination of CRISPR/Cas9 and somatic cell nuclear transfer. We also report a

SUBMITTER: Ruan J 

PROVIDER: S-EPMC4585612 | biostudies-literature | 2015 Sep

REPOSITORIES: biostudies-literature