Cryo-EM and the elucidation of new macromolecular structures: Random Conical Tilt revisited.

ABSTRACT: Cryo-Electron Microscopy (cryo-EM) of macromolecular complexes is a fundamental structural biology technique which is expanding at a very fast pace. Key to its success in elucidating the three-dimensional structure of a macromolecular complex, especially of small and non-symmetric ones, is the ability to start from a low resolution map, which is subsequently refined with the actual images collected at the microscope. There are several methods to produce this first structure. Among them, Random Conical Tilt (RCT) plays a prominent role due to its unbiased nature (it can create an initial model based on experimental measurements). In this article, we revise the fundamental mathematical expressions supporting RCT, providing new expressions handling all key geometrical parameters without the n

SUBMITTER: Sorzano CO 

PROVIDER: S-EPMC4585738 | biostudies-literature | 2015 Sep

REPOSITORIES: biostudies-literature