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QIAD assay for quantitating a compound's efficacy in elimination of toxic A? oligomers.

ABSTRACT: Strong evidence exists for a central role of amyloid ?-protein (A?) oligomers in the pathogenesis of Alzheimer's disease. We have developed a fast, reliable and robust in vitro assay, termed QIAD, to quantify the effect of any compound on the A? aggregate size distribution. Applying QIAD, we studied the effect of homotaurine, scyllo-inositol, EGCG, the benzofuran derivative KMS88009, ZA?3W, the D-enantiomeric peptide D3 and its tandem version D3D3 on A? aggregation. The predictive power of the assay for in vivo efficacy is demonstrated by comparing the oligomer elimination efficiency of D3 and D3D3 with their treatment effects in animal models of Alzheimer´s disease.

SUBMITTER: Brener O

PROVIDER: S-EPMC4585794 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature

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QIAD assay for quantitating a compound's efficacy in elimination of toxic Aβ oligomers.

Brener Oleksandr O Dunkelmann Tina T Gremer Lothar L van Groen Thomas T Mirecka Ewa A EA Kadish Inga I Willuweit Antje A Kutzsche Janine J Jürgens Dagmar D Rudolph Stephan S Tusche Markus M Bongen Patrick P Pietruszka Jörg J Oesterhelt Filipp F Langen Karl-Josef KJ Demuth Hans-Ulrich HU Janssen Arnold A Hoyer Wolfgang W Funke Susanne A SA Nagel-Steger Luitgard L Willbold Dieter D

Scientific reports 20150923

Strong evidence exists for a central role of amyloid β-protein (Aβ) oligomers in the pathogenesis of Alzheimer's disease. We have developed a fast, reliable and robust in vitro assay, termed QIAD, to quantify the effect of any compound on the Aβ aggregate size distribution. Applying QIAD, we studied the effect of homotaurine, scyllo-inositol, EGCG, the benzofuran derivative KMS88009, ZAβ3W, the D-enantiomeric peptide D3 and its tandem version D3D3 on Aβ aggregation. The predictive power of the a ...[more]

PMID: 26394756

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