Protein interaction patterns in different cellular environments are revealed by in-cell NMR.

ABSTRACT: In-cell NMR allows obtaining atomic-level information on biological macromolecules in their physiological environment. Soluble proteins may interact with the cellular environment in different ways: either specifically, with their functional partners, or non-specifically, with other cellular components. Such behaviour often causes the disappearance of the NMR signals. Here we show that by introducing mutations on the human protein profilin 1, used here as a test case, the in-cell NMR signals can be recovered. In human cells both specific and non-specific interactions are present, while in bacterial cells only the effect of non-specific interactions is observed. By comparing the NMR signal recovery pattern in human and bacterial cells, the relative contribution of each type of interaction ca

SUBMITTER: Barbieri L 

PROVIDER: S-EPMC4585868 | biostudies-literature | 2015 Sep

REPOSITORIES: biostudies-literature