Interleukin-6 mediates enhanced thrombus development in cerebral arterioles following a brief period of focal brain ischemia.

ABSTRACT:

Objective

The cerebral microvasculature is rendered more vulnerable to thrombus formation following a brief (5.0 min) period of focal ischemia. This study examined the contribution of interleukin-6 (IL-6), a neuroprotective and prothrombotic cytokine produced by the brain, to transient ischemia-induced thrombosis in cerebral arterioles.

Approach & results

The middle cerebral artery of C57BL/6J mice was occluded for 5 min, followed by 24h of reperfusion (MCAo/R). Intravital fluorescence microscopy was used to monitor thrombus development in cerebral arterioles induced by light/dye photoactivation. Thrombosis was quantified as the time of onset of platelet aggregation on the vessel wall and the time for complete blood flow cessation. MCAo/R in wild type (WT) mice yielded an ac

SUBMITTER: Tang YH 

PROVIDER: S-EPMC4586404 | biostudies-literature | 2015 Sep

REPOSITORIES: biostudies-literature