Comparison of methods for the enumeration of enterohemorrhagic Escherichia coli from veal hides and carcasses.

ABSTRACT: The increased association of enterohemorrhagic Escherichia coli (EHEC) with veal calves has led the United States Department of Agriculture Food Safety and Inspection Service to report results of veal meat contaminated with the Top 7 serogroups separately from beef cattle. However, detection methods that can also provide concentration for determining the prevalence and abundance of EHEC associated with veal are lacking. Here we compared the ability of qPCR and a molecular based most probable number assay (MPN) to detect and enumerate EHEC from veal hides at the abattoir and the resulting pre-intervention carcasses. In addition, digital PCR (dPCR) was used to analyze select samples. The qPCR assay was able to enumerate total EHEC in 32% of the hide samples with a range of approximately 34 t

SUBMITTER: Luedtke BE 

PROVIDER: S-EPMC4586433 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature