Optimization of CLARITY for Clearing Whole-Brain and Other Intact Organs

ABSTRACT: The development, refinement, and use of techniques that allow high-throughput imaging of whole brains with cellular resolution will help us understand the complex functions of the brain. Such techniques are crucial for the analysis of complete neuronal morphology-anatomical and functional-connectivity, and repeated molecular phenotyping. CLARITY is a recently introduced technique that produces structurally intact, yet optically transparent tissue, which may be labeled and imaged without sectioning. However, the utility of this technique depends on several procedural variables during the process in which the light-scattering lipids in a tissue are replaced by a transparent hydrogel matrix. Here, we systematically varied a number of factors (including temperature, hydrogel composition, and p

SUBMITTER: Epp JR 

PROVIDER: S-EPMC4586927 | biostudies-literature | 2015 May-Jun

REPOSITORIES: biostudies-literature