Beyond genomics: critical evaluation of cell line utility for ovarian cancer research.

ABSTRACT:

Objective

Comparisons of The Cancer Genome Atlas (TCGA) with high grade serous ovarian cancer (HGSOC) cell lines used in research reveal that many common experimental models lack defining genomic characteristics seen in patient tumors. As cell lines exist with higher genomic fidelity to TCGA, this study aimed to evaluate the utility of these cell lines as tools for preclinical investigation.

Methods

We compared two HGSOC cell lines with supposed high genomic fidelity to TCGA, KURAMOCHI and OVSAHO, with the most commonly cited ovarian cancer cell line, SKOV3, which has poor genomic fidelity to TCGA. The lines were analyzed for genomic alterations, in vitro performance, and growth in murine xenografts.

Results

Using targeted next generation sequencing analyses, we deter

SUBMITTER: Elias KM 

PROVIDER: S-EPMC4587360 | biostudies-literature | 2015 Oct

REPOSITORIES: biostudies-literature