Highly multiplexed imaging of single cells using a high-throughput cyclic immunofluorescence method.

ABSTRACT: Single-cell analysis reveals aspects of cellular physiology not evident from population-based studies, particularly in the case of highly multiplexed methods such as mass cytometry (CyTOF) able to correlate the levels of multiple signalling, differentiation and cell fate markers. Immunofluorescence (IF) microscopy adds information on cell morphology and the microenvironment that are not obtained using flow-based techniques, but the multiplicity of conventional IF is limited. This has motivated development of imaging methods that require specialized instrumentation, exotic reagents or proprietary protocols that are difficult to reproduce in most laboratories. Here we report a public-domain method for achieving high multiplicity single-cell IF using cyclic immunofluorescence (CycIF), a simpl

SUBMITTER: Lin JR 

PROVIDER: S-EPMC4587398 | biostudies-literature | 2015 Sep

REPOSITORIES: biostudies-literature