Chromatin interaction analysis reveals changes in small chromosome and telomere clustering between epithelial and breast cancer cells.

ABSTRACT:

Background

Higher-order chromatin structure is often perturbed in cancer and other pathological states. Although several genetic and epigenetic differences have been charted between normal and breast cancer tissues, changes in higher-order chromatin organization during tumorigenesis have not been fully explored. To probe the differences in higher-order chromatin structure between mammary epithelial and breast cancer cells, we performed Hi-C analysis on MCF-10A mammary epithelial and MCF-7 breast cancer cell lines.

Results

Our studies reveal that the small, gene-rich chromosomes chr16 through chr22 in the MCF-7 breast cancer genome display decreased interaction frequency with each other compared to the inter-chromosomal interaction frequency in the MCF-10A epithelial cells. I

SUBMITTER: Barutcu AR 

PROVIDER: S-EPMC4587679 | biostudies-literature | 2015 Sep

REPOSITORIES: biostudies-literature