Cultivation of stable, reproducible microbial communities from different fecal donors using minibioreactor arrays (MBRAs).

ABSTRACT:

Background

Continuous-flow culture models are one tool for studying complex interactions between members of human fecal microbiotas because they allow studies to be completed during an extended period of time under conditions where pH, nutrient availability, and washout of waste products and dead cells can be controlled. Because many of the existing well-validated continuous-flow models are large and complex, we were interested in developing a simpler continuous-flow system that would allow microbial community dynamics to be examined in higher throughput while still maintaining complex microbial communities. To this end, we developed minibioreactor arrays (MBRAs), small volume bioreactors (15 ml) that allow simultaneous cultivation of up to 48 microbial communities in a single anae

SUBMITTER: Auchtung JM 

PROVIDER: S-EPMC4588258 | biostudies-literature | 2015 Sep

REPOSITORIES: biostudies-literature