Unraveling the regulatory network of IncA/C plasmid mobilization: When genomic islands hijack conjugative elements.

ABSTRACT: Conjugative plasmids of the A/C incompatibility group (IncA/C) have become substantial players in the dissemination of multidrug resistance. These large conjugative plasmids are characterized by their broad host-range, extended spectrum of antimicrobials resistance, and prevalence in enteric bacteria recovered from both environmental and clinical settings. Until recently, relatively little was known about the basic biology of IncA/C plasmids, mostly because of the hindrance of multidrug resistance for molecular biology experiments. To circumvent this issue, we previously developed pVCR94ΔX, a convenient prototype that codes for a reduced set of antibiotic resistances. Using pVCR94ΔX, we then characterized the regulatory pathway governing IncA/C plasmid dissemination. We found that the expr

SUBMITTER: Carraro N 

PROVIDER: S-EPMC4588540 | biostudies-literature | 2015 May-Jun

REPOSITORIES: biostudies-literature