WormGender - Open-Source Software for Automatic Caenorhabditis elegans Sex Ratio Measurement.

ABSTRACT: Fast and quantitative analysis of animal phenotypes is one of the major challenges of current biology. Here we report the WormGender open-source software, which is designed for accurate quantification of sex ratio in Caenorhabditis elegans. The software functions include, i) automatic recognition and counting of adult hermaphrodites and males, ii) a manual inspection feature that enables manual correction of errors, and iii) flexibility to use new training images to optimize the software for different imaging conditions. We evaluated the performance of our software by comparing manual and automated assessment of sex ratio. Our data showed that the WormGender software provided overall accurate sex ratio measurements. We further demonstrated the usage of WormGender by quantifying the high in

SUBMITTER: Labocha MK 

PROVIDER: S-EPMC4589236 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature