Analysis of a high-throughput cone-and-plate apparatus for the application of defined spatiotemporal flow to cultured cells.

ABSTRACT: The shear stresses derived from blood flow regulate many aspects of vascular and immunobiology. In vitro studies on the shear stress-mediated mechanobiology of endothelial cells have been carried out using systems analogous to the cone-and-plate viscometer in which a rotating, low-angle cone applies fluid shear stress to cells grown on an underlying, flat culture surface. We recently developed a device that could perform high-throughput studies on shear-mediated mechanobiology through the rotation of cone-tipped shafts in a standard 96-well culture plate. Here, we present a model of the three-dimensional flow within the culture wells with a rotating, cone-tipped shaft. Using this model we examined the effects of modifying the design parameters of the system to allow the device to create a

SUBMITTER: Spruell C 

PROVIDER: S-EPMC4589279 | biostudies-literature | 2013 Jun

REPOSITORIES: biostudies-literature