Proteomic mapping of ER-PM junctions identifies STIMATE as a regulator of Ca²⁺ influx.

ABSTRACT: Specialized junctional sites that connect the plasma membrane (PM) and endoplasmic reticulum (ER) play critical roles in controlling lipid metabolism and Ca(2+) signalling. Store-operated Ca(2+) entry mediated by dynamic STIM1-ORAI1 coupling represents a classical molecular event occurring at ER-PM junctions, but the protein composition and how previously unrecognized protein regulators facilitate this process remain ill-defined. Using a combination of spatially restricted biotin labelling in situ coupled with mass spectrometry and a secondary screen based on bimolecular fluorescence complementation, we mapped the proteome of intact ER-PM junctions in living cells without disrupting their architectural integrity. Our approaches led to the discovery of an ER-resident multi-transmembrane pro

SUBMITTER: Jing J 

PROVIDER: S-EPMC4589512 | biostudies-literature | 2015 Oct

REPOSITORIES: biostudies-literature