Nano-mole scale sequential signal assignment by (1)H-detected protein solid-state NMR.

ABSTRACT: We present a 3D (1)H-detected solid-state NMR (SSNMR) approach for main-chain signal assignments of 10-100 nmol of fully protonated proteins using ultra-fast magic-angle spinning (MAS) at ∼80 kHz by a novel spectral-editing method, which permits drastic spectral simplification. The approach offers ∼110 fold time saving over a traditional 3D (13)C-detected SSNMR approach.

SUBMITTER: Wang S 

PROVIDER: S-EPMC4589527 | biostudies-literature | 2015 Oct

REPOSITORIES: biostudies-literature