Collagenase IV plays an important role in regulating hair cycle by inducing VEGF, IGF-1, and TGF-β expression.

ABSTRACT:

Background

It has been reported that collagenases (matrix metalloproteinase 2 [MMP-2] and matrix metalloproteinase 9 [MMP-9]) are associated with hair cycle, whereas the mechanism of the association is largely unknown.

Methods

The mice were randomly allocated into four groups: saline, and 5, 10, and 15 nM SB-3CT. Immunohistochemical analysis was employed to examine MMP-2 and MMP-9 protein. Real-time polymerase chain reaction and enzyme-linked immunosorbent assay were performed to determine mRNA and protein levels of VEGF, IGF-1, TGF-β, and GAPDH. Growing hair follicles from anagen phase III-IV were scored based on hematoxylin and eosin staining. Hair regrowth was also evaluated.

Results

Results showed that mRNA expressions of enzymes changed with a peak at late anagen

SUBMITTER: Hou C 

PROVIDER: S-EPMC4590320 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature