Programming cells by multiplex genome engineering and accelerated evolution.

ABSTRACT: The breadth of genomic diversity found among organisms in nature allows populations to adapt to diverse environments. However, genomic diversity is difficult to generate in the laboratory and new phenotypes do not easily arise on practical timescales. Although in vitro and directed evolution methods have created genetic variants with usefully altered phenotypes, these methods are limited to laborious and serial manipulation of single genes and are not used for parallel and continuous directed evolution of gene networks or genomes. Here, we describe multiplex automated genome engineering (MAGE) for large-scale programming and evolution of cells. MAGE simultaneously targets many locations on the chromosome for modification in a single cell or across a population of cells, thus producing comb

SUBMITTER: Wang HH 

PROVIDER: S-EPMC4590770 | biostudies-literature | 2009 Aug

REPOSITORIES: biostudies-literature