Novel approaches for two and three dimensional multiplexed imaging of osteocytes.

ABSTRACT: Although osteocytes have historically been viewed as quiescent cells, it is now clear that they are highly active cells in bone and play key regulatory roles in diverse skeletal functions, including mechanotransduction, phosphate homeostasis and regulation of osteoblast and osteoclast activity. Three dimensional imaging of embedded osteocytes and their dendritic connections within intact bone specimens can be quite challenging and many of the currently available methods are actually imaging the lacunocanalicular network rather than the osteocytes themselves. With the explosion of interest in the field of osteocyte biology, there is an increased need for reliable ways to image these cells in live and fixed bone specimens. Here we report the development of reproducible methods for 2D and 3D

SUBMITTER: Kamel-ElSayed SA 

PROVIDER: S-EPMC4591054 | biostudies-literature | 2015 Jul

REPOSITORIES: biostudies-literature