A Comparison of transgenic and wild type soybean seeds: analysis of transcriptome profiles using RNA-Seq.

ABSTRACT:

Background

Soybean (Glycine max) has been bred for thousands of years to produce seeds rich in protein for human and animal consumption, making them an appealing bioreactor for producing valuable recombinant proteins at high levels. However, the effects of expressing recombinant protein at high levels on bean physiology are not well understood. To address this, we investigated whether gene expression within transgenic soybean seed tissue is altered when large amounts of recombinant proteins are being produced and stored exclusively in the seeds. We used RNA-Seq to survey gene expression in three transgenic soybean lines expressing recombinant protein at levels representing up to 1.61 % of total protein in seed tissues. The three lines included: ST77, expressing human thyroglobulin

SUBMITTER: Lambirth KC 

PROVIDER: S-EPMC4591623 | biostudies-literature | 2015 Oct

REPOSITORIES: biostudies-literature