Role of Dectin-1 in the innate immune response of rat corneal epithelial cells to Aspergillus fumigatus.

ABSTRACT:

Background

To observe Dectin-1 expression in fungal keratitis on rat models and to determine the role of Dectin-1 in innate immune response to Aspergillus fumigatus.

Methods

Wistar rats were randomly divided into control, fungal keratitis and pretreatment (pretreated with Laminarin) groups. Samples were used for conducting immunohistochemical staining and real-time PCR to observe expression of cytokines like CCL2, CCL3, CXCL1, CXCL2, IL-1β, TNF-α, IL-6, IL-10.

Results

After fungal stimulations, all 7 inflammatory factors, except IL-10, increased with different levels. After 4 h of fungal stimulations, IL-1β, IL-6, CCL2, CXCL1 and CXCL2 of pretreatment groups were significantly (p < 0.05) lower than fungal groups, while the other 3 cytokines had no significant changes.

SUBMITTER: Xu Q 

PROVIDER: S-EPMC4591637 | biostudies-literature | 2015 Oct

REPOSITORIES: biostudies-literature