Host-Associated Metagenomics: A Guide to Generating Infectious RNA Viromes.

ABSTRACT:

Background

Metagenomic analyses have been widely used in the last decade to describe viral communities in various environments or to identify the etiology of human, animal, and plant pathologies. Here, we present a simple and standardized protocol that allows for the purification and sequencing of RNA viromes from complex biological samples with an important reduction of host DNA and RNA contaminants, while preserving the infectivity of viral particles.

Principal findings

We evaluated different viral purification steps, random reverse transcriptions and sequence-independent amplifications of a pool of representative RNA viruses. Viruses remained infectious after the purification process. We then validated the protocol by sequencing the RNA virome of human body lice engorged

SUBMITTER: Temmam S 

PROVIDER: S-EPMC4592258 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature