DNA Methylation Predicts Progression of Human Gastric Lesions.

ABSTRACT:

Background

Development of the intestinal subtype of gastric adenocarcinoma is marked by a progression of histopathologic lesions. Residents of the Andean regions of Colombia are at high risk for gastric cancer.

Methods

A cohort of 976 Colombian subjects was followed over 16 years examining effects of Helicobacter pylori eradication and treatment with antioxidants on progression of lesions. We performed methylation analysis of DNA from baseline antral biopsies from 104 subjects for whom follow-up data were available for at least 12 years. Methylation was quantitated for AMPH, CDKN2A, CDH1, EN1, EMX1, NKX6-1, PCDH10, RPRM, RSPO2, SORCS3, ZIC1, and ZNF610 genes, using Pyrosequencing.

Results

Levels of DNA methylation were associated with baseline diagnosis for AMPH, EMX1

SUBMITTER: Schneider BG 

PROVIDER: S-EPMC4592454 | biostudies-literature | 2015 Oct

REPOSITORIES: biostudies-literature