Integrated glycoprotein immobilization method for glycopeptide and glycan analysis of cardiac hypertrophy.

ABSTRACT: Post-translational modifications of proteins can have a major role in disease initiation and progression. Incredible efforts have recently been made to study the regulation of glycoproteins for disease prognosis and diagnosis. It is essential to elucidate glycans and intact glycoproteins to understand the role of glycosylation in diseases. Sialylated N-glycans play crucial roles in physiological and pathological processes; however, it is laborious to study sialylated glycoproteins due to the labile nature of sialic acid residues. In this study, an integrated platform is developed for the analysis of intact glycoproteins and glycans using a chemoenzymatic approach for immobilization and derivatization of sialic acids. N-Glycans, deglycosylated proteins, and intact glycoproteins from heart t

SUBMITTER: Yang S 

PROVIDER: S-EPMC4592484 | biostudies-literature | 2015 Oct

REPOSITORIES: biostudies-literature