Improved OTU-picking using long-read 16S rRNA gene amplicon sequencing and generic hierarchical clustering.

ABSTRACT:

Background

High-throughput bacterial 16S rRNA gene sequencing followed by clustering of short sequences into operational taxonomic units (OTUs) is widely used for microbiome profiling. However, clustering of short 16S rRNA gene reads into biologically meaningful OTUs is challenging, in part because nucleotide variation along the 16S rRNA gene is only partially captured by short reads. The recent emergence of long-read platforms, such as single-molecule real-time (SMRT) sequencing from Pacific Biosciences, offers the potential for improved taxonomic and phylogenetic profiling. Here, we evaluate the performance of long- and short-read 16S rRNA gene sequencing using simulated and experimental data, followed by OTU inference using computational pipelines based on heuristic and complete

SUBMITTER: Franzen O 

PROVIDER: S-EPMC4593230 | biostudies-literature | 2015 Oct

REPOSITORIES: biostudies-literature